Akhilesh Kumar developed a method to determine the efficiency of genome editing using different guidernas. This method is called "dna modificationgenome editing." This technology is used in many fields such as in therapeutics, crop improvement, and fundamental research. Crisprcas9 is a recently developed genome editing technology which has revolutionized the genome editing scenario. This technology uses an enzyme called "cas9" and a guiderna. Cas9 cuts the target DNA, and the guiderna identifies and binds to the target DNA. Then, the cas9 enzyme cuts the DNA at the desired location. Once the DNA is cut, the cell's repair mechanism can be used to add, delete, or change the desired sequence in the genome. Emmanuelle Charpentier and Jennifer Doudna received the Nobel Prize in Chemistry in 2020 for inventing this technology.
